Table 5.
Cytokinin levels inArabidopsis thalianaextracts determined by UHPLC-ESI(+)MS/MS
|
CKs |
Cytokinin content (pmol g–1FW) |
|||
|---|---|---|---|---|
| Seedlings | Shoots | Roots | Root segments | |
|
tZ |
n.d. |
n.d. |
0.96 ± 0.12 |
2.60 ± 0.97 |
|
tZR |
2.23 ± 0.37 |
0.88 ± 0.32 |
3.27 ± 0.52 |
4.92 ± 0.94 |
|
tZ7G |
37.99 ± 2.81 |
23.49 ± 3.07 |
6.54 ± 0.75 |
n.d. |
|
tZ9G |
3.88 ± 1.22 |
4.32 ± 1.31 |
2.08 ± 0.52 |
n.d. |
|
tZOG |
9.25 ± 2.77 |
9.42 ± 1.75 |
7.10 ± 1.33 |
7.63 ± 2.45 |
|
tZMP |
n.d. |
n.d. |
n.d. |
8.04 ± 2.44 |
|
cZ |
n.d. |
n.d. |
n.d. |
0.95 ± 0.33 |
|
cZR |
0.80 ± 0.16 |
1.14 ± 0.37 |
3.71 ± 0.79 |
2.28 ± 0.41 |
|
cZ9G |
n.d. |
n.d. |
2.02 ± 0.47 |
n.d. |
|
cZOG |
0.90 ± 0.27 |
1.04 ± 0.33 |
2.89 ± 0.66 |
3.35 ± 0.39 |
| DHZR |
0.64 ± 0.19 |
0.94 ± 0.27 |
0.97 ± 0.28 |
n.d. |
| DHZ7G |
5.48 ± 1.28 |
5.78 ± 1.51 |
2.07 ± 0.42 |
n.d. |
| DHZ9G |
n.d. |
0.71 ± 0.22 |
0.29 ± 0.07 |
n.d. |
| DHZOG |
0.46 ± 0.13 |
0.28 ± 0.07 |
0.15 ± 0.05 |
n.d. |
| iP |
0.24 ± 0.10 |
0.15 ± 0.04 |
0.56 ± 0.17 |
0.42 ± 0.14 |
| iPR |
1.96 ± 0.28 |
1.24 ± 0.26 |
2.18 ± 0.46 |
5.15 ± 0.60 |
| iP7G |
53.87 ± 2.86 |
65.76 ± 12.47 |
30.16 ± 4.57 |
n.d. |
| iPMP | n.d. | n.d. | n.d. | 13.14 ± 0.35 |
Values are meansf ± SD (n = 12); n.d. – not detected.
10-day-old A. thaliana seedlings, roots and shoots and apical part of the root of 8-day-old plants (approximately 0.5 cm) were extracted in Bieleski buffer, purified by multi-StageTip microcolumns and measured by UHPLC-ESI(+)-MS/MS method.