Figure 1.
Constructs PJR-ADH and PRD-ADH. A, General map of the T-DNA region of the binary vector Bin 19 (Bevan, 1984; Frisch et al., 1995) showing the Npt II-selectable marker gene and the polylinker site. B, For construction of the vector PJR-ADH, the tomato Adh 2 cDNA was ligated between the CaMV 35S promoter (prom) and the nopaline synthase (Nos) 3′ terminator, which were inserted into the polycloning site of the Bin 19 vector as described in Methods. C, For construction of the PRD-ADH vector, the tomato Adh 2 cDNA was ligated between the promoter and 3′ regions of the tomato PG, which were inserted into the polycloning site of the Bin 19 vector as described in Methods.
