Table I.

Number of introduced genes, ADH specific activities, and relative abundances of some volatiles in fruit from transformed and control plants

Plant	Genes Inserted (Adh 2/Np II)	ADH Specific Activity	Hexanal	Z-3-Hexanal	E-2-Hexanal	Hexanol	Z-3-Hexanol	Geraniol	E-2-Octenal	1-Octen-3-ol	Citral
		units mg−1 protein	relative abundance
C7 (n = 4)	1/1	0.67  ± 0.16a	8.48  ± 1.60b	8.42  ± 1.07b	14.38  ± 3.24b	0.25  ± 0.09b	3.08  ± 0.28a	0.72  ± 0.34	0.43  ± 0.01	0.09  ± 0.01	0.30  ± 0.10
C14 (n = 3)	1/1	0.56  ± 0.04c	9.13  ± 0.31b	7.28  ± 0.40b	13.57  ± 1.76b	0.40  ± 0.05c	4.12  ± 0.79b	0.71  ± 0.16	0.50  ± 0.09	0.07  ± 0.02	0.90  ± 0.14
C20 (n = 5)	2/2	0.76  ± 0.15c	9.94  ± 1.98b	7.73  ± 1.28b	15.99  ± 1.72c	0.52  ± 0.17a	6.02  ± 1.58c	0.62  ± 0.14	0.57  ± 0.06	0.11  ± 0.02	0.69  ± 0.28
C13 (n = 3)	6/7	1.26  ± 0.31a	6.95  ± 0.89a	7.67  ± 1.42b	12.92  ± 2.78b	0.29  ± 0.08b	4.48  ± 1.27b	0.49  ± 0.10	0.45  ± 0.03	0.08  ± 0.01	0.38  ± 0.10
AC1–3 (n = 10)	–	0.375  ± 0.15	9.43  ± 1.44	7.76  ± 1.57	12.37  ± 2.03	0.22  ± 0.07	2.51  ± 0.46	0.89  ± 0.25	0.61  ± 0.09	0.10  ± 0.03	0.70  ± 0.22
C4 (n = 3)	4/3	0.01  ± 0.016d	9.62  ± 0.85b	9.28  ± 1.22b	11.68  ± 2.62b	0.02  ± 0.025d	0.15  ± 0.08d	0.81  ± 0.13	0.56  ± 0.1	0.12  ± 0.03	0.6  ± 0.01
C11 (n = 4)	8/?	0.006  ± 0.01d	8.45  ± 0.44b	7.57  ± 0.72b	15.88  ± 1.42c	0.04  ± 0.02d	0.065  ± 0.08d	0.6  ± 0.09	0.52  ± 0.05	0.10  ± 0.02	0.35  ± 0.07
C23 (n = 3)	3/5	0.011  ± 0.02d	10.09  ± 2.27b	8.71  ± 2.46b	10.82  ± 3.23b	0.02  ± 0.002d	0.057  ± 0.05d	0.92  ± 0.15	0.54  ± 0.20	0.09  ± 0.03	0.44  ± 0.15

The number of inserted genes was estimated by quantitating the hybridization to genomic DNA of probes for Npt II and for the Adh 2 cDNA, as detailed in Methods. Headspace volatiles were extracted from Br+7 fruit, collected by SPME absorption, and analyzed by GC-MS. Peak areas were determined by integration and were normalized against the area of a deuterated hexanol standard introduced during maceration of the tissue. Averaged ADH activities from pericarp tissue and volatile abundances were derived from the same sets of fruit, and were derived separately from the data presented in Figure 2. Data from individual transformed plants were compared with pooled data from the control plants (AC1, 2, and 3) using Welch's t test, which does not assume equal variances: Statistical analyses of geraniol, E-2-octenal, 1-octen-3-ol, and citral are not included because no correlation with ADH activity was evident.

^aSignificant (P < 0.05). 

^bNot significant. 

^cVery significant (P < 0.01). 

^dExtremely significant (P < 0.001).