Table II.
Parent T0 | No. of Inserts | No. of T1 Analyzed | T1 Segregationa |
---|---|---|---|
C13 | 6 | 16 | 3/1, 4/3, 6/3, 7/4, 8/3, 12/2 |
C4 | 4 | 39 | 0/2, 1/9, 2/1, 3/2, 4/10, 5/6, 6/3, 7/2, 8/4 |
C11 | 8 | 40 | 0/7, 6/3, 7/3, 8/5, 9/6, 10/3, 11/2, 12/3, 13/2, 14/3, 16/3 |
C23 | 3 | 30 | 0/1, 1/9, 2/10, 3/3, 4/3, 5/2, 6/2 |
The number of transgene inserts in the T1 plants was determined by Southern-blot analysis and quantitation of number and intensity of bands of hybridization relative to hybridization to the endogenous genes.
No. of inserts/no. of T1.