Table 1.
PCR primer sequences,G6PDgene fragments amplified and PCR product sizes
| Frag-ment | Exon(s) | Forward primer sequence (5’-3’) | Reverse primer sequence (5’-3’) | PCR product size |
|---|---|---|---|---|
| 1 |
1,2 |
CAATAGGGCCGGCTTGAC |
TGCAACAATTAGTTGGAAAAGC |
1376bp |
| 2 |
3,4,5 |
TGTCCCCAGCCACTTCTAAC |
CTCATAGAGTGGTGGGAGCA |
1125bp |
| 3 |
6,7 |
AGGGGTTCAAGGGGGTAAC |
TGCAGGGTGACTGGCTCT |
563bp |
| 4 |
8 |
ACAGGGCGGGGAGCTAAG |
GTGCCTCGTCACAGATGG |
202bp |
| 5 |
9,10 |
CCTGAGGGCTGCACATCT |
GTGTCTTGCTGATGCCACTG |
741bp |
| 6 | 11,12,13 | TGGCATCAGCAAGACACTCT | GACAAGGAAGTGGGTCCTCA | 1151bp |