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. 2012 Aug 29;12:377. doi: 10.1186/1471-2407-12-377

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Copyright ©2012 Shimoda et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of gene expression related to EMT and CSCs by real-time PCR. The mRNA expression levels of the indicated EMT-related genes (A) and embryonic stem cell markers (Nodal, Pax6, Rex1, Lefty, Oct-4, and Nanog) and differentiation markers (mesoderm marker: Brachyury; ectoderm marker: Sox2; endoderm marker: AFP) (B) in ACC cells were quantified by real-time RT-PCR. Each mRNA level was compared between ACCS GFP and ACCS-M GFP, and data are shown as relative mRNA levels to β-actin mRNA. Experiments were performed in triplicate, and the number of adhered cells was averaged. Bars indicate the standard deviation.