Figure 4.

Silencing of Brachyury recovers the epithelial signature of ACCS-M GFP cells. sh control (sh. con) cells were generated by the transfection of ACCS GFP or ACCS-M GFP cells with control vector. sh Brachyury (sh.Bra) or sh SOX2 (sh.SOX2) cells were generated by the transfection of ACCS GFP or ACCS-M GFP cells with Brachyury shRNA or SOX2 shRNA. Cells were cultured for 24 h on culture dishes, and cell lysates were prepared and resolved using 10% SDS-PAGE. The levels of EMT-related biomarkers were detected by immunoblotting with antibodies against the indicated proteins. All experiments were performed at least in triplicate, and representative results are shown.