Table 1.

Analysis of gene expression levels related to EMT and CSCs by real-time PCR

Genes	Cell line	Relative mRNA level (normalized to β-actin)
		Mean expression levels	SD
Snail	ACCS	2.02 × 10-2	4.18 × 10-3
	ACCSM	5.14 × 10-2	2.62 × 10-3
Slug	ACCS	1.05 × 10-2	4.82 × 10-3
	ACCSM	4.14 × 10-2	2.92 × 10-3
Twist1	ACCS	1.47 × 10-4	5.24 × 10-5
	ACCSM	3.37 × 10-4	1.12 × 10-5
Twist2	ACCS	4.68 × 10-3	2.67 × 10-4
	ACCSM	8.04 × 10-3	1.38 × 10-4
Zeb1	ACCS	3.21 × 10-3	5.13 × 10-4
	ACCSM	2.92 × 10-2	6.16 × 10-4
Zeb2	ACCS	1.40 × 10-3	6.23 × 10-4
	ACCSM	7.39 × 10-3	4.12 × 10-4
Tgf-β2	ACCS	5.43 × 10-2	3.56 × 10-2
	ACCSM	1.81 × 10-1	7.52 × 10-2
Gsk3β	ACCS	3.39 × 10-3	1.82 × 10-4
	ACCSM	8.73 × 10-3	6.43 × 10-4
Nodal	ACCS	3.72 × 10-3	4.36 × 10-4
	ACCSM	7.14 × 10-3	2.15 × 10-4
Pax 6	ACCS	6.34 × 10-2	4.26 × 10-2
	ACCSM	1.10 × 10-1	8.21 × 10-2
Rex 1	ACCS	1.12 × 10-3	2.42 × 10-4
	ACCSM	2.45 × 10-3	2.93 × 10-4
Lefty	ACCS	3.39 × 10-3	1.51 × 10-4
	ACCSM	9.88 × 10-3	7.12 × 10-4
Brachyury	ACCS	1.55 × 10-2	5.32 × 10-3
	ACCSM	2.83 × 10-2	6.24 × 10-3
Sox 2	ACCS	9.35 × 10-3	2.12 × 10-4
	ACCSM	1.94 × 10-2	8.92 × 10-4
AFP	ACCS	9.50 × 10-3	3.52 × 10-4
	ACCSM	1.83 × 10-2	9.96 × 10-4