Table 3.
Concentration of the different hordein coding DNA clones
|
Hordein primers |
DNA concentration (amol/μl) |
|
|---|---|---|
| |
Added DNA* |
Calculated from Ctvalues |
| Pooled all (15): B1 (5), B3 (4), C (1), D (2), γ1 (2) and γ3 (1) | ||
|
Common B |
5.16 |
5.71 ± 0.18 |
|
B1 |
2.94 |
2.92 ± 0.11 |
|
B1a |
2.94 |
2.74 ± 0.55 |
|
JQ867084 |
0.63 |
0.54 ± 0.14 |
|
B3 |
2.21 |
2.41 ± 0.61 |
|
B3a |
2.21 |
1.81 ± 0.11 |
|
B3b |
0.88 |
1.09 ± 0.02 |
|
Common C |
0.53 |
0.51 ± 0.02 |
|
Common D |
0.43 |
0.51 ± 0.01 |
|
Common γ1 |
1.04 |
0.97 ± 0.07 |
|
Common γ3 |
0.54 |
0.56 ± 0.00 |
| JQ867080 | 0.54 | 0.47 ± 0.07 |
*DNA concentration was measured for individual clones and the numbers represent amol/μl DNA in the pool.
Note: the calculation based on three replicates and presented as means ± SD.
B1-hordein clones: JQ859916, JQ859917, JQ867073, JQ867074, JQ867084; B3-hordein clones: JQ867085, JQ867086, JQ867087, JQ867089; C-hordein clone: JQ867090; D-hordein clones: JQ867076, JQ867077; γ1-hordein clones: JQ867078, JQ867079; γ3-hordein clone: JQ867080.