Table 4.
Stability of selected housekeeping genes in the developing barley grain
| DAP | Actin/Ubiquitin | Actin/PTF | Ubiquitin/Actin | Ubiquitin/PTF | PTF/Actin | PTF/Ubiquitin |
|---|---|---|---|---|---|---|
|
10 |
0.16 ± 0.01 |
0.12 ± 0.01 |
6.15 ± 0.19 |
0.72 ± 0.02 |
8.56 ± 0.33 |
1.39 ± 0.05 |
|
15 |
0.13 ± 0.03 |
0.17 ± 0.04 |
7.53 ± 0.48 |
1.26 ± 0.08 |
6.57 ± 0.23 |
0.79 ± 0.14 |
|
18 |
0.14 ± 0.03 |
0.20 ± 0.04 |
6.17 ± 0.93 |
1.42 ± 0.34 |
5.99 ± 0.24 |
0.71 ± 0.24 |
| 25 | 0.11 ± 0.00 | 0.08 ± 0.00 | 9.21 ± 0.32 | 0.70 ± 0.02 | 13.19 ± 0.38 | 1.44 ± 0.04 |
Ratio: difference in the amount of the two housekeeping genes; calculated as amol x 10-3.