Figure 2. The tyrosine phosphatase Shp2 controls the down-regulation of Bim.

(A) Knocking-down of Shp2 in 606HS2 and 931HS2 cells. Whole cell extracts were immunoblotted using antibodies raised against Shp2, P-Erk1/2, Erk1/2, Bim and the cleaved form of caspase-3. (B) Overexpression of Shp2 in 606HS2 and 931HS2 cells. Whole cell extracts were immunoblotted using indicated antibodies. The expression of Shp2^WT-MT and Shp2 ^C459S-MT was detected as the band above the endogenous Shp2. (C) Whole cell extracts from 606HS2 and 931HS2 cells treated or not (–) for 4 h with PI3K inhibitor NVP-BEZ235 (120 nM) were immunoblotted using indicated antibodies. (D) Knocking-down of Stat5 in 606HS2 and 931HS2 cells. Whole cell extracts were immunoblotted using anti-Bim, anti-Stat5 antibodies and anti-β actin antibody as loading control.