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. 2012 Nov 7;7(11):e49037. doi: 10.1371/journal.pone.0049037

Figure 3. Influence of m-dcps in respiratory electron transport chains of mixotrophic Scenedesmus obliquus cultures.

Figure 3

(A) Respiration activity in isolated mitochondria treated with m-dcps in reduced form (with ascorbate) (dark grey), rotenone + m-dcps in reduced form (grey), rotenone + antimycin + m-dcps in reduced form (light grey). (B) Respiration rate (measured polarographically as consumption of oxygen) in Scenedesmus obliquus cultures treated with m-dcps after five incubation days. (dark grey) Total respiration rate, (grey) COX capacity (SHAM and PG used), (light grey) AOX capacity (KCN and PG used) and (white) PTOX activity (KCN and SHAM used). (C) Short-term effect of m-dcps on mitochondrial potential, (dark grey) after 1 min incubation with m-dcp and JC-1, (grey) after 10 min incubation with m-dcp and JC-1, (light grey) after 30 min incubation with m-dcp and JC-1 and (white) after 1 h incubation with m-dcp and JC-1. (D) Western blot analysis of immunoreactive COX, AOX and PTOX proteins in the fifth incubation day with m-dcps (the same pattern observed in the second incubation day with m-dcps). (E) Long-term effect of m-dcps on mitochondrial potential after 5 days incubation with each m-dcp in the hermitical closed bottles. (F) Fluorescence microscopy of cells incubated with m-dcps for five days in the hermitical closed bottles.