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. 2012 Nov 7;7(11):e48810. doi: 10.1371/journal.pone.0048810

Figure 4. HHV-6B binds to the sperm head.

Figure 4

Semen samples were incubated with HHV-6B for two hrs, washed twice in PBS and stained with anti-gp60/110 or anti-IgG2b followed by a goat-anti mouse (GaM)-FITC. (A-B) and (E-K) HHV-6B binds specifically to the front region of the sperm head. (C) Control staining for non-specific binding of anti-gp60/110. Sperm were pre-incubated in vehicle and stained with anti-gp60/110 and GaM-FITC. (D) Control staining for non-specific binding of GaM-FITC. Sperm were pre-incubated with HHV-6B and stained with IgG2b and GaM-FITC.