Skip to main content
. 2012 Nov 7;7(11):e48809. doi: 10.1371/journal.pone.0048809

Figure 2. Physicochemical characteristics of sfGFP (black line) in comparison with EGFP (gray line).

Figure 2

(A) Absorption spectra in UV- and visible spectra regions, (inset to A) CD in visible-UV region. (B) CD in far-UV and near-UV (inset to B) regions. (C) Intrinsic fluorescence spectra at excitation wavelength of 297 nm. (D) Green chromophore fluorescence of both sfGFP and EGFP at excitation wavelength of 390 nm (solid black and gray lines, respectively). Fluorescence spectrum of sfGFP at excitation wavelength of 295 nm (dotted line) is also shown. Fluorescence spectra of sfGFP at excitation wavelengths of 390 nm (solid line), 485 nm (dashed line), corresponding to absorption of neutral and anionic chromophore, are presented on inset to D.