Table 3. Characteristics of the Trp57 microenvironment in sfGFP.

N	residue	atoms	R *, Å
1	Asp216	OD1(NE1)	2.82
2	Asp216	OD2(NE1)	4.12
3	Lys209	NZ(CD1)	3.91
4	Met218	SD(CE3)	4.18
5	Tyr143	OH(CG)	5.31
6	Cys48	SG(CZ2)	5.65
7	HOH	(CD1)	3.57
8	HOH	(CD1)	3.67
9	HOH	(CD1)	3.95
10	HOH	(NE1)	4.21
11	HOH	(CD1)	3.90
12	HOH	(NE1)	5.32
13	Phe46	CG	4.62
14	Tyr143	CE1	4.45
15	Pro56	CA	5.45
16	Pro58	CD	4.8

^* R is the minimal distance between a residue involved in the microenvironment of tryptophan residue and an indole ring.

Residues 1–12 are polar groups of the Trp57 microenvironment.

Residues 13–16 are aromatic residues of the Trp57 microenvironment.