Table 2. 24 hour energy expenditure (EE) and respiratory quotient (RQ) in ShcKO and wild-type (WT) mice housed at 22°C or 12°C.¹ .

	P66 Shc(−/−)			WT			P Value
	3 mo	15 mo	27 mo	3 mo	15 mo	27 mo	Geno	Age	Geno*Age
22°C
RQ	0.921±0.011	0.904±0.004	0.939±0.015	0.917±0.008	0.883±0.004	0.906±0.006	0.050	0.067	0.912
RQMEI α	0.919±0.010	0.908±0.013	0.938±0.010	0.914±0.007	0.885±0.012	0.909±0.007	0.020	0.051	0.912
EE (kJ/mouse)	40.072±0.664a	43.475±1.398b	39.406±0.922a	42.722±0.993a	47.943±1.108b	43.846±1.465a	0.0003	0.0047	0.685
EEBW (kJ)¥	42.173±1.001a	41.218±1.194a	38.577±0.812b	47.024±1.174a	44.240±1.606a	40.301±1.094b	0.012	0.0001	0.929
EEFFM (kJ)$	42.822±1.409a	41.277±1.374a	37.816±1.064b	45.997±1.566a	44.979±2.121a	41.195±1.434b	0.115	0.0009	0.822
EEORGAN (kJ)+	42.439±1.413a	42.848±1.072a	38.782±0.890b	46.593±1.833a	45.506±1.806a	42.451±1.041b	0.006	0.002	0.495
12°C
RQ	0.907±0.013	0.886±0.003	0.888±0.007	0.897±0.009	0.882±0.003	0.883±0.006	0.412	0.077	0.506
RQMEI α	0.908±0.009a	0.883±0.010b	0.888±0.008ab	0.896±0.005a	0.877±0.009b	0.887±0.005ab	0.173	0.039	0.687
EE (kJ/mouse)	49.866±0.897a	53.558±1.523b	51.347±1.163ab	53.431±1.769a	60.262±1.688b	57.547±1.916ab	0.0002	0.0195	0.311
EEBW (kJ)¥	51.004±1.513	52.338±1.695	51.149±1.091	58.531±2.106	56.689±2.598	54.204±1.694	0.005	0.595	0.098
EEFFM (kJ)$	52.518±2.019	51.826±1.691	50.144±1.297	60.574±2.363	55.066±2.653	52.929±2.653	0.076	0.064	0.284
EEORGAN (kJ)+	51.472±2.044	53.511±1.412	51.300±1.166	59.046±2.327	56.521±2.068	55.592±1.175	0.009	0.498	0.208

MEI, metabolizable energy intake; BW, body weight; FFM, fat-free mass; ORGAN, crude organ mass (sum of liver, kidney, heart, and brain mass); EE is expressed as EE kJ/mouse (kJ per mouse) and EE_{BW, FFM, ORGAN} (kJ normalized by BW, FFM, and ORGAN).

¹Data are presented as means ± SEM unless otherwise indicated; superscript letters that differ indicate differences between ages within temperature and genotype, Bonferroni corrected P values provided in table;