Table 3. Energy expenditure and respiratory quotient in ShcKO and wild-type (WT) mice housed at 22°C.¹ .

	ShcKO			WT			P Value
	3 mo	15 mo	27 mo	3 mo	15 mo	27 mo	Geno	Age	Geno*Age
Fasted
RQ	0.831±0.018a	0.796±0.0030b	0.808±0.011b	0.0823±0.009a	0.779±0.0053b	0.793±0.0053b	0.180	0.005	0.921
RQMEI α	0.829±0.0122a	0.798±0.014b	0.808±0.0115b	0.819±0.0078a	0.779±0.0126b	0.796±0.0074b	0.179	0.046	0.931
EE (kJ/min/mouse)	0.0241±0.0005a	0.0267±0.00045b	0.0240±0.00091ab	0.026±0.00011a	0.028±0.0011b	0.027±0.00090ab	0.002	0.032	0.728
EEBW (kJ/min)¥,	0.0259±8.42E-4a	0.0251±8.70E-4ab	0.0235±5.93E-4b	0.0290±1.27E-4a	0.0261±1.44E-3ab	0.0247±9.89E-4b	0.068	0.004	0.873
EEFFM (kJ/min)$	0.027±9.52E-4a	0.024±7.97E-4ab	0.022±6.11E-4b	0.028±1.58E-3a	0.026±1.77E-3ab	0.025±1.20E-3b	0.341	0.0002	0.888
EEORGAN (kJ/min)+	0.0261±1.18E-3a	0.262±5.98E-4ab	0.236±5.88E-4a	0.028±1.99E-3a	0.027±1.04E-3ab	0.026±8.59E-4a	0.021	0.033	0.639
Fed
RQ	1.016±0.011a	0.998±0.0068a	1.051±0.019b	1.006±0.010a	0.979±0.0053a	1.002±0.00935b	0.016	0.040	0.289
RQMEI α	1.01±0.013a	1.00±0.0159a	1.05±0.013b	0.999±0.009a	0.981±0.014a	1.01±0.008b	0.010	0.009	0.506
EE (kJ/min/mouse)	0.0316±0.0012a	0.0331±0.0016b	0.0301±0.005a	0.0340±0.0013a	0.0375±0.00067b	0.0333±0.0011a	0.002	0.025	0.549
EEBW (kJ/min)¥,	0.033±1.42E-3a	0.031±1.46E-3a	0.029±1.00E-3b	0.037±9.61E-4a	0.034±1.09E-3a	0.030±7.47E-4b	0.064	0.0005	0.453
EEFFM (kJ/min)$	0.032±2.21E-3a	0.033±2.48E-3a	0.030±1.26E-3b	0.037±1.34E-3a	0.035±1.51E-3a	0.031±1.02E-3b	0.155	0.004	0.618
EEORGAN (kJ/min)+	0.033±2.25E-3a	0.033±1.59E-3a	0.030±5.98E-4b	0.037±1.31E-3a	0.035±9.13E-4a	0.032±7.96E-4b	0.035	0.006	0.690

MEI, metabolizable energy intake; BW, body weight; FFM, fat-free mass; ORGAN, crude organ mass (sum of liver, kidney, heart, and brain mass); EE is expressed as EE kJ/min/mouse (kJ per min per mouse) and EE_{BW, FFM, ORGAN} (kJ/min normalized by BW, FFM, and ORGAN).

¹Data are presented as means ± SEM unless otherwise indicated; superscript letters that differ indicate differences between ages within temperature and feed period, Bonferroni corrected P values provided in table;

^{α, ¥, $,+}values are presented as least square mean ± SEM, adjusted for MEI, BW, FFM, and ORGAN, respectively.