Figure 4. Whole-mount in situ hybridization of the myogenic regulatory factor myoD in cranial and trunk muscle precursors at 48 hpf.

Wild type siblings (a,a'); unc45a^−/− (b,b'); unc45b^−/− (c,c'); and unc45b^−/−; unc45a^−/− (d,d') mutants. Compared to wild type siblings (a) myoD expression in the cranial muscle precursors is unchanged in the unc45a^−/− (b), unc45b^−/− (c), and unc45b^−/−; unc45a^−/− (d) mutants. unc45b^−/− (c') and unc45b^−/−; unc45a^−/− (d') mutants have increased expression of myoD in the trunk precursors compared to wild type siblings (a') and unc45a^−/− mutants (b'). Primes denote alternative views of the same embryo. chv, constrictor hyoideus ventralis; im, intermandibularis; ir, inferior rectus; lr, lateral rectus; mr, medial rectus; pam, pharyngeal arch muscles; sh, sternohyoideus; so, superior oblique; sr, superior rectus.