Figure 2. Molecular characterization of AmAChE1 and AmAChE2 by polyacrylamide gel electrophoresis and Western blot analysis.

Protein samples were extracted from the heads of forager honey bees with 0.1 M Tris-HCl buffer in the presence or absence of 0.5% Triton X-100 to determine the soluble nature of the AmAChEs (A). To investigate the GPI-anchor properties of the AmAChEs, protein samples were treated with PIPLC (B). Protein samples were mixed with or without β-mercaptoethanol and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis to determine the multimer formation of AmAChE1 and AmAChE2 (C).