Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
NL4-3 (WT) plasmid was used to generate the NL4-3/ΔRT plasmid by deleting an 892 bp fragment, which codes for the retrotranscriptase (RT) and is inside the pol gene. These plasmids were used to transfect 293-T cells in order to obtain viruses. Numbers refer to pNL4-3.
