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. 2012 Nov 7;7(11):e49301. doi: 10.1371/journal.pone.0049301

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A. Immunoprecipitation with anti-PARP-1 antibody from untreated (N/T) and MMS-treated cell extracts with the repair times specified. B. Immunoprecipitation with anti-Ku70 antibody as in A. Immunoblotting (IB) was performed with the antibodies specified. An IgG negative control was performed for both immunoprecipitations (lanes 7), and 30 µg of cell extract (input wild-type and pol β null, respectively) was used as a source of marker proteins (lanes 8 and 9).