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. 2012 Nov 7;7(11):e49341. doi: 10.1371/journal.pone.0049341

Figure 6. Comparative analysis of HERV-Ec1 gag and PLA2G4A transcription in patients with urothelial carcinoma.

Figure 6

QRT-PCR assays were performed on DNA-free RNA samples obtained from patients with urothelial carcinoma (n = 11). Due to lack of material, additional patients were used not shown in Figures 1 and 2. For measurement of HERV-Ec1 transcripts locus-specific primers derived from the gag region of HERV-Ec1 were utilized. Transcript levels of PLA2G4A were analyzed using a gene specific primer set. Relative transcription levels were normalized to G6PD transcript levels and represent the mean value of triplicate qRT-PCR assays. The numbers on the Y-axis show the fold change of transcription in urothelial carcinoma with respect to corresponding mean value of non-malignant tissue samples (value = 1, depicted by red horizontal line). Thus, values >1 denote increases, values <1 correspond to decreases in transcript levels.