Figure 3. Tissue viability analysis.
(A) Viability index of the epidermal sheet determined by CCK-8 assay. There is no significant difference in the viability index between the 8- and 10-h digestion groups, while the value of the 12-h digestion group is significantly lower than that of the 8-h digestion group. n = 5. (B) The percentage of viable cells detected by Hoe/PI staining. With the duration of Dispase II digestion prolonging, the percentage of viable cells in the epidermal sheet decreases gradually. n = 5.