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. 2012 Nov 7;7(11):e49428. doi: 10.1371/journal.pone.0049428

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A. Schematic diagram of Cbl-c constructs used to map the interaction with Hic-5. All constructs were HA-epitope tagged on the N-terminus. B. HEK293T cells were transfected with various HA- Cbl-c constructs and Hic-5 as indicated above panels. Immunoprecipitates (IP) or cell lysates (lysates) were immunoblotted (IB) as indicated to the right of the panels. All transfections were balanced with empty vector controls. GFP was transfected as a control for transfection efficiency and shown as a control for loading. MWs in kDa are shown to the left of the panels.