Figure 6. Histochemical analysis of GUS activity during seed development in Arabidopsis lines harbouring transgenes FPS1S::GUS (panels A to E) and FPS2::GUS (panels F to J).

Seeds containing embryos at the globular (A and F), heart (B and G), early-torpedo (C and H), torpedo (D and I) and maturation green (E and J) developmental stages were analyzed for GUS expression as described under Material and Methods. GUS expression in the root meristematic region of the embryo at heart (G) and early-torpedo (H) stages in FPS2::GUS seeds is indicated by arrowheads. The inset between panels (H) and (I) shows expression of FPS2::GUS in the root meristematic region and the pro-vascular tissue of an embryo at the early-torpedo stage.