Table 1. Steady-state kinetic constants for FPS1S and FPS2.
Variable substrate | Kinetic parameter | FPS1S | FPS2 |
IPP | K m (μM) | 23.1±0.4 | 31.3±0.5 |
kcat (min−1) | 62.5 | 269.0 | |
kcat/K m (min−1μM−1) | 2.7 | 8.6 | |
DMAPP | K m (μM) | 22.7±2.1 | 31.5±0.5 |
kcat (min−1) | 52.2 | 197.3 | |
kcat/K m (min−1μM−1) | 2.3 | 6.2 | |
GPP | K m (μM) | 8.3±1.2 | 11.3±1.0 |
kcat (min−1) | 36.4 | 132.8 | |
kcat/K m (min−1μM−1) | 4.4 | 11.7 |
The K m mean values and SE were calculated from three independent experiments.
kcat values were calculated for the dimeric form of the enzyme.