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. 2012 Nov 7;7(11):e49201. doi: 10.1371/journal.pone.0049201

Figure 1. cDNA library screening identifies EML4-ALK.

Figure 1

A. Screening of a library generated from H2228 cells generated 16 clones with distinct patterns of integrated cDNA. All clones contained one of two EML4-ALK transcripts that differed based on splicing of exon 6 and the length of the 5′ untranslated region, as indicated by the most 5′ nucleotide fused to the attB sequence. λ indicates size ladder. B. Screening of a library generated from H3122 cells generated Ba/F3 clones isolated at the indicated time point after withdrawal of IL3. PCR is shown using primers that amplify integrated cDNA. C. PCR amplification from eight clones generated from the H3122 library with different patterns of integrated cDNA. Five distinct EML4-ALK transcripts were identified based on differences in the length of the 5′ untranslated region, as in A.