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. 2012 Nov 7;7(11):e49201. doi: 10.1371/journal.pone.0049201

Figure 2. cDNA library screening identified an ALK translocation partner and resistance allele.

Figure 2

A. Immunohistochemistry using an antibody against ALK and karyotype of the anaplastic large cell lymphoma specimen 421. B. Multiple IL3-independent clones were recovered from screening of the cDNA library generated from 421, all of which contained ALK cDNA using primers within the flanking attB sites or within ALK itself. C. Immunoblot using antibody against ALK demonstrates ATIC-ALK expression in multiple clones but not in wild-type Ba/F3 cells. D. Effect on proliferation of 96 individual clones recovered from the 421 library with TAE684 300 nM, quantified using the ATP chemiluminescent agent CellTiterGlo. E. PCR amplification using the att-f and att-r primers with DNA from clone D3 demonstrates a full-length STAT5B allele that harbored a G210T (K70N) mutation.