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. 2012 Nov 7;7(11):e49201. doi: 10.1371/journal.pone.0049201

Figure 5. Recovery of a transforming ERRB2 splice variant.

Figure 5

A. cDNA library screening of the melanoma cell lines 70 W (a sub-line of the MeWo) and HCC1569 resulted in IL3 independent Ba/F3 clones that contained ERBB2Δ16. B. Relative proliferation quantified using the ATP chemiluminescent agent CellTiterGlo. C. cDNA transcripts recovered from screening of MeWo, HCC1569 and 70 W cell lines. All of the transcripts lacked amino acids 2137–2184 that comprise exon 6. D. Expression of ERBB2 cDNA containing exon 16 using specific primers. Error bars indicate standard deviation. E. Expression of ERBB2Δ16 cDNA using specific primers, including a 5′ primer that overlaps exons 15 and 17 so does not amplify wild-type ERBB2.