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. 2012 Nov 7;7(11):e49186. doi: 10.1371/journal.pone.0049186

Figure 2. BLT2 is required for the invasiveness of MDA-MB-231 and MDA-MB-435 breast cancer cells.

Figure 2

(A and B) MDA-MB-231 (A) and MDA-MB-435 (B) cells were transfected with BLT2 (siBLT2) or control (siCont) siRNAs for 48 h in medium supplemented with 0.5% FBS, after which the amounts of BLT1 and BLT2 mRNAs were determined by semiquantitative RT-PCR (left panel) and quantitative real-time PCR (center and right panels) analysis. Data are representative of five independent experiments. (C) Cells treated as in (A) and (B) were assayed for invasiveness. Representative invading cells stained with H&E are shown, and quantitative data are expressed relative to the value for cells transfected with the control siRNA. Scale bars, 50 µm. (D) MDA-MB-231 and MDA-MB-435 cells were incubated with U75302 (0.5 or 1 µM), LY255283 (5 or 10 µM), or DMSO vehicle for 30 min and were then assayed for invasiveness in the continued presence of the corresponding test agent. Scale bars, 50 µm. All quantitative data are means±SD from five independent experiments. *P<0.05, †P<0.01, ‡P<0.005.