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. 2012 Nov 7;7(11):e48329. doi: 10.1371/journal.pone.0048329

Figure 2. Schematic representation of the experimental protocol.

Figure 2

The center channels were always used for one reagent at a time. Step 1: 0.5 µL of blocking buffer (BB) was injected from all the inlets and incubated for 3 min. Step 2: 0.5 µL of the target miRNA sustained in BB, 1 µL of biotinylated probe DNA (10 nM in the BB), and 0.5 µL of BB, were injected from the left, center, and right inlets, respectively. Step 3: After 5 min of incubation, 3 µL of F-SA (2.5 µg/mL in BB), 6 µL of B-anti-SA (25 µg/mL in BB), and 3 µL of F-SA (2.5 µg/mL in BB) were injected from the left, center, and right inlets, respectively.