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. 2012 Nov 7;7(11):e48262. doi: 10.1371/journal.pone.0048262

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© 2012 Kresse et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Plot of the DNA methylation status and mRNA expression level of CXCL5 based on methylation-specific PCR and quantitative real-time RT-PCR, respectively, in five cell lines and five tumour samples. The mRNA expression levels have been normalised to the expression of the house-keeping gene GAPDH and then to the average expression level of the two normal osteoblast samples. The DNA methylation status is indicated with coloured circles; black, full methylation, grey, partial methylation. The DNA methylation and mRNA expression levels based on the microarray data are indicated for the cell lines; N, normal methylation; H, hyper-methylation; U, under-expression.