Skip to main content
. 2012 Nov 7;7(11):e48381. doi: 10.1371/journal.pone.0048381

Figure 1. Workflow and tools for Fluorescence Behavioral Imaging (FBI). A).

Figure 1

Workflow of FBI experiments. Experiments are performed using an FBI hardware system, sQuid software for multi-camera acquisition/LED control, and Actin88F:eGFP transgenic Drosophila melanogaster. Subsequently, infrared movies are processed using Ctrax [2] tracking software. FBI post-processing scripts then employ tracking data and fluorescence images to determine the genetic identity of behaving flies in an automated fashion. B) Bright-field (top) and fluorescence (bottom) images of an Actin88F:eGFP female fly. C) FBI hardware system used in this paper. D) Illumination during a single FBI experiment. FBI requires infrared backlight and blue fluorescence illumination following each experiment (solid blocks). Infrared and blue fluorescence illumination prior to each experiment and/or green illumination for vision-dependent behaviors are optional (hatched blocks).