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. 2012 Nov 7;7(11):e48416. doi: 10.1371/journal.pone.0048416

Table 2. 25 polymorphic SNPs identified during DNA sequencing of 40 controls and 10 cases.

Gene Loci Genotype Frequency In Our Study (%) CA Trend Fishers-Exact
According To rs MOR AA Homozygous Homozygous Test Test
SNP MOR11 Number Transcript Change Patients Major3 Heterozygote3 Minor3 p-value4 MAF p-value4
1 5′UR/T-2694G 2 rs12210856 - Cases 90.00 10.00 - 0.197 5.00 0.46
Controls 70.00 30.00 - 15.00
2 5′UR/A-2455C rs12190259 - Cases 90.00 10.00 - 0.197 5.00 0.46
Controls 70.00 30.00 - 15.00
3 5′UR/G-1509A rs12205732 - Cases 90.00 10.00 - 0.197 5.00 0.46
Controls 70.00 30.00 - 15.00
4 5′UTR/G-172T rs6912029 MOR1 Cases 90.00 10.00 - 0.156 5.00 0.29
Controls 67.50 32.50 - 16.25
5 E1/A118G rs1799971 MOR1 Asn→Asp Cases 30.00 70.00 - 0.643 35.00 0.80
Controls 32.50 55.00 12.50 40.00
6 I1/G-2994A rs563649 MOR1K1 Cases 90.00 10.00 - 0.247 5.00 0.45
5′UTR Controls 72.50 27.50 - 13.75
7 I1/G -2259A rs9322446 MOR1K1 Cases 90.00 10.00 - 1.000 5.00 1.00
5′UTR Controls 90.00 10.00 - 5.00
8 E3/G877A NV1 MOR1 Val→Ile Cases 100.00 0.00 - 0.471 0.00 1.00
Controls 95.00 5.00 - 2.50
9 I3/G399T NV2 MOR1A Cases 90.00 10.00 - 0.043 5.00 0.20
3′UTR Controls 100.00 0.00 - 0.00
10 I3/A1839T rs540825 MOR1X Glu→His Cases 60.00 40.00 - 0.002 * (0.035 * ) 20.00 0.01 * (0.15)
E4 Controls 95.00 5.00 - 2.50
11 I3/C1956T rs675026 MOR1X Gly→Gly Cases 60.00 30.00 10.00 0.030* (0.456) 25.00 0.04* (0.60)
E4 Controls 87.50 10.00 2.50 7.50
12 I3/A1966G rs562859 MOR1X Leu→Leu Cases 70.00 20.00 10.00 0.103 20.00 0.11
E4 Controls 87.50 10.00 2.50 7.50
13 I3/C-11152T NV3 MOR1B5 Glu→Stop Cases 60.00 40.00 - 0.042* (0.633) 20.00 0.05
Controls 92.50 5.00 2.50 5.00
14 I3/C18957T rs606545 MOR1B1 Cases 60.00 40.00 - 0.042* (0.633) 20.00 0.05
3′UTR Controls 92.50 5.00 2.50 5.00
15 3′UTR/A725G rs17181352 MOR1 Cases 100.00 0.00 - 0.614 0.00 1.00
Controls 97.50 2.50 - 1.25
16 3′DR/A148G rs671531 - Cases 80.00 20.00 - 0.459 10.00 0.60
Controls 92.50 5.00 2.50 5.00
17 3′DR/A633G rs583664 - Cases 70.00 30.00 - 0.158 15.00 0.14
Controls 92.50 5.00 2.50 5.00
18 3′DR/C932T rs658156 - Cases 60.00 40.00 - 0.042* (0.633) 20.00 0.05
Controls 92.50 5.00 2.50 5.00
19 3′DR/G971A rs9371776 - Cases 100.00 - - 0.372 - 1.00
Controls 92.50 7.50 - 3.75
20 3′DR/G1270A rs558948 - Cases 60.00 40.00 - 0.042* (0.633) 20.00 0.05
Controls 92.50 5.00 2.50 5.00
21 3′DR/T1371C rs558025 - Cases 60.00 40.00 - 0.042* (0.633) 20.00 0.05
Controls 92.50 5.00 2.50 5.00
22 3′DR/C1510T rs645027 - Cases 80.00 20.00 - 0.44 10.00 0.73
Controls 67.50 32.50 - 16.25
23 3′DR/C1549T rs598160 - Cases 60.00 40.00 - 0.042* (0.633) 20.00 0.05
Controls 92.50 5.00 2.50 5.00
24 3′DR/C1657G rs644261 - Cases 60.00 40.00 - 0.042* (0.633) 20.00 0.05
Controls 92.50 5.00 2.50 5.00
25 3′DR/G1665A NV4 - Cases 100.00 - - 0.614 - 1.00
Controls 97.50 2.50 - 1.25

Footnote: SNP 10, rs540825, was significantly associated with fentanyl-induced emesis pre- and post- Bonferroni correction.

Abbreviations: SNP: Single nucleotide polymorphism; UR: Upstream region; DR: Downstream region;

UTR: Untranslated region; E: Exon; I: Intron; NV: Novel variant; MOR: Mu opioid receptor;

NCBI: National Centre For Biotechnology Information; CA: Cochran-Armitage; AA: Amino acid;

MAF: Minor allele frequency; Asn: Asparigine; Asp: Aspartate; Val: Valine; Ile: Isoleucine;

Glu: Glutamine; His: Histidine; Gly: Glycine; Leu: Leucine.

Legends:

1

MOR1 is the main transcript of OPRM1. Other transcripts of MOR1 e.g. MOR1X and MOR1K1 are splice variants of MOR.

2

The letter in capitals refers to the minor allele of the SNP.

3

Patients with 2 copies of the major or minor allele respectively. Heterozygote: Patients with 1 copy each of the major and minor allele.

4

Non-bracketed p-values for the CA trend test and Fishers-Exact test refers to p-values before Bonferroni correction. Bracketed (p-values) refers to p-values post-Bonferroni correction.

*

p-value<0.05 indicates statistical significance.