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. 2012 Nov 7;7(11):e47039. doi: 10.1371/journal.pone.0047039

Figure 7. HPLC-SEC profiles (fluorescence emission detection) of O:2-ADH-SIDEA-CRM197 (dotted line; 28.7 µg/mL of protein; Kd of 0.39) in comparison to free CRM197 (dashed line; 50 mg/mL; Kd of 0.69) and free O:2 (solid line; 1 mg/mL of sugar; Kd of 0.55).

Figure 7

80 µL of each samples run on TosoHaas TSK gel 6000+5000 PW columns; eluent: 0.1 M NaH2PO4, 0.1 M NaCl, 5% CH3CN, pH 7.2; flow rate: 0.5 mL/min. Column void volume: 23.65 min.; total volume: 49.07 min. Free O:2 is not detected by fluorescence emission.