Table 3. Characterization of the conjugates used in the immunogenicity study in mice.

Conjugate	Total sugar, µg/mL	Total protein, µg/mL	wt/wt ratio O:2 to CRM197	Kd (HPLC-SEC)
O:2-(CDAP)ADH-CRM197	32.88	62.98	0.52	0.44
O:2-CDH-SIDEA-CRM197	82.82	36.67	2.26	0.40
O:2-ADH-SIDEA-CRM197	51.54	29.56	1.74	0.39
O:2-(EDAC)ADH-CRM197	50.19	31.31	1.60	0.52
O:2				0.55
CRM197				0.69