Table 4. O:2 chain sugar composition remains unchanged during conjugation steps to produce O:2-ADH-SIDEA-CRM197.
Sugar composition, molar ratio | ||||
by 1H NMR | by HPAEC-PAD | |||
Sample | Par/Rha | Man/Rha | Gal/Rha | Glc/Rha |
Underivatized O:2 | 1.06 | 1.07 | 1.05 | 0.74 |
O:2-ADH | 1.08 | 1.05 | 0.99 | 0.73 |
O:2-ADH-SIDEA | 1.08 | 1.05 | 0.98 | 0.83 |
O:2-ADH-SIDEA-CRM197 | 1.05 | 1.04 | 1.07 | 0.83 |