Table 5. Characterization of conjugates after purification on Sephacryl S-300 HR column.

Conjugate	Total sugar, µg/mL	Total protein, µg/mL	Wt/wt ratio O:2 toCRM197	Kd (HPLC-SEC)
O:2-ADH-SIDEA-CRM197	51.54	29.56	1.74	0.39
O:2-SIDEA-CRM197	108.6	52.8	2.06	0.38
O:2-(TNBS)ADH-SIDEA-CRM197	54.8	23.7	2.31	0.44