Figure 5.

Immunoblots of AOX in rapidly extracted whole-root samples from 4-, 7-, and 17-d-old soybean seedlings. Samples treated in the presence and absence of DTT (50 mm) and diamide (5 mm) are presented. For each age, lanes are from a single gel, and each lane shown was separated by two empty lanes to avoid cross-contamination of redox chemicals. The intensity of the reaction at 35 and 75 kD was measured using National Institutes of Health imaging software. At a given root age, the 35-kD band in the DTT lanes had the highest density of all bands present in the three lanes, and this was arbitrarily set at 1.0; the densities of the other bands in those lanes are presented as fractions of that highest-density band.