Figure 3. A3V gene transduction in post-hatch chick brain.
(A–C) EGFP expression 1 week after A3V injection (a total of 5×107, 5×108, and 5×109 GC, respectively) was analyzed by immunofluorescence labeling of parasagittal sections. Arrowheads indicate injection sites in the striatum. Scale bars are 1 mm. (D) Gene transduction after LV or A3V injection was quantified by measurements of EGFP-expressing area in the parasagittal sections containing injection sites (n = 4). The raw data are listed in Table S4. (E) A3V-treated striatal cells were visualized by double immunofluorescence labeling for EGFP (green) and neuronal marker NeuN (red). Scale bar indicates 50 µm. (F) EGFP expression 24 hrs after A3V injection (a total of 5×109 GC) into the intermediate medial mesopallium (IMM) was analyzed by immunofluorescence labeling of coronal sections. The arrowhead indicates the A3V injection site. Scale bar is 1 mm.