Figure 3.

Effect of chrysin on PMA-induced MUC5AC gene expression from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of chrysin for 30 minutes and were then stimulated with PMA (10 ng/mL) for 24 hours. MUC5AC gene expression was measured by RT-PCR. Rig/S15 rRNA, which encodes a small ribosomal subunit protein, a housekeeping gene that was constitutively expressed, was used as a quantitative control. PMA: phorbol 12-myristate 13-acetate; RT-PCR: reverse transcription polymerase chain reaction; Cont: control; Chr: chrysin. Concentration unit is µM.