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A - Fluorogenic substrates with different specificities for cathepsin-like proteases were incubated with extracts of L. (L.) chagasi amastigotes in the presence of DTT and increasing concentrations of RF07, as indicated. B and C - Fluorogenic substrate specific for cathepsin B-like proteases was incubated with extracts of L. (L.) chagasi in presence of increasing concentrations of either RF07 (B) or CA074 (C).
