Table 3. Effect of treatment with 10 μM MG132 from 0–6 or 16–22 h of maturation on subsequent embryonic development (Experiment 3).^a .

MG132,0–6 h	MG132, 16–22 h		No. of oocytes	Percentage of oocytes developing to			Percentage of cleaved embryos developing to the blastocyst stage	No. of cells in blastocyst
				≥2-cell		Blastocyst
No	No		164	58.8±2.3b		22.4±1.9b	38.0±2.4b	142.4±2.2b
No	Yes		143	68.4±2.3c		34.8±1.9c	49.3±2.4c	148.7±2.2b
Yes	No		161	34.5±2.3d		10.9±1.9d	29.9±2.4bd	141.3±2.5b
Yes	Yes		166	41.0±2.3d		10.2±1.9d	24.1±2.4d	144.1±2.2b
Probability of treatment effectse
MG132, 0–6				0.001	0.01		0.01	N.S.
MG132, 16–22				0.05	N.S.		N.S.	P<0.02
Interaction				N.S.	0.05		0.05	N.S.

^aData are least-squares means ± SEM of values from six replicates.

^b,c,dValues in the same column with different superscript letters are significantly different (P<0.05).

^eN.S. = non-significant (P>0.10).