Table 5. Effect of treatment with 10 μM MG132 from 0–6 or 16–22 h of maturation on meiotic maturation at 22 h after initiation of maturation (Experiment 5).a .
MG132, 0–6 h | MG132, 16–22 h | No. of oocytes | Nuclear status, % b | ||||
GVBD | MI | Ana-Telo | MII | ||||
No | No | 91 | 0.9±1.2c | 14.2±0.8c | 0.0±2.9c | 84.9±3.5c | |
No | Yes | 79 | 0.0±1.2c | 19.1±0.8d | 6.0±2.9c | 74.8±3.5c | |
Yes | No | 69 | 0.0±1.2c | 34.8±0.8e | 3.2±2.9c | 62.0±3.5c | |
Yes | Yes | 69 | 1.6±1.2c | 34.7±0.8e | 3.6±2.9c | 60.2±3.5c | |
Probability of treatment effectsf | |||||||
MG132, 0–6 | N.S. | N.S. | N.S. | N.S. | |||
MG132, 16–22 | N.S. | N.S. | N.S. | N.S. | |||
Interaction | N.S. | P<0.09 | N.S. | N.S. |
Data are least-squares means ± SEM of values from three replicates.
GVBD: germinal vesicle break down; MI: metaphase I; Ana-Telo: anaphase – telophase; MII: metaphase II.
Values in the same column with different superscript letters are significantly different (P<0.05 or, for).
N.S. = non-significant (P>0.10).