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. 2012 Nov 7;7(11):e48574. doi: 10.1371/journal.pone.0048574

Figure 1. Gal-9 secretion in naïve CD4 T cell culture upon TCR stimulation.

Figure 1

(A) Naïve CD4 T cells were cultured in Th17-skewing conditions, neutral conditions (TCR stimulation), or without stimulation for 96 h before released Gal-9 was measured using ELISA. (B) Same as (A), except that the cells were cultured under Th17-skewing conditions, neutral conditions, neutral conditions plus TGF-β1 (TGF-β1 alone), or neutral conditions plus IL-6 (IL-6 alone). (C) Gal-9 mRNA was quantified using real-time RT-PCR. (D) Gal-9 secretion in the presence of human stable Gal-9 (30 nM) was measured. The human protein was not detected by mouse Gal-9 ELISA (see text for details). All results are shown as the mean ± SEM of quadruplicates. p<0.001 (***), p<0.05 (*), not significant (NS). Representative data out of at least 2 experiments are shown.