Figure 1. The expression of shS1/FliC rather than shS1 or FliC enhances the levels and duration of proinflammatory cytokine production by DCs.

Murine BMDCs were transduced with the recombinant Ad vectors at an MOI of 250. A, 24 h later, culture media were collected for evaluation of the representative cytokines by ELISA. B, cells were analyzed for expression of costimulatory and MHC class-II molecules by flow cytometry. C, 24 h later, cultures were washed and replaced with fresh medium that does not contain stimuli. The concentrations of representative cytokines in culture medium 3 days after washing were examined by ELISA. Data are representative of three repeated experiments. *p<0.01; **p<0.05.