Figure 3. Tetracycline inhibiting the DNA binding activity of NF-κB.

The binding activity of of NF-κB was assessed by Electrophoretic mobility shift assay (EMSA). (A) EMSA autoradiography of NF-κB DNA binding activity. (B) Levels of NF-κB DNA binding activity expressed as arbitrary densitometric units (A.U.) using Image J. Compared to the control group, NF-κB binding activity was significantly decreased in the tetracycline group. Bars represent mean±SD; *P<0.05 versus control group.