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. 2012 Nov 7;7(11):e48672. doi: 10.1371/journal.pone.0048672

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© 2012 Jiang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The mRNA and protein were detected by ELISA and real time PCR respectively. (A) Both tetracycline and 3-MA significantly decreased the levels of TNF-α. (B) IL6 was significantly decreased by tetracycline and 3-MA. (C) Tetracycline down-regulated mRNA expressions of TNF-α. (B) The mRNA level of IL6 in the brain was inhibited by tetracycline and 3-MA. Bars represent mean±SD (n = 6); *P<0.05 vs. control group.