Figure 3. Interaction between CnMVs and HBMECs.

(A) HBMECs were incubated with purified CnMVs and then examined by confocal microscopy. The HBMECs were stained with FITC-conjugated anti-GFP (A1), rhodamine-conjugated anti-CD44 (A2), and DAPI (A3). An overlaid image of A1, A2 and A3 is shown in A4. The yellow arrows indicate faint, diffuse staining in the cytoplasm of HBMECs. Bar: 20 µm. (B) HBMECs were incubated with JB02 C. neoformans cells and then examined by confocal microscopy. The HBMECs were stained with FITC-conjugated anti-GFP (B1), rhodamine-conjugated anti-CD44 (B2), and DAPI (B3). An overlaid image of B1, B2 and B3 is shown in B4. Bar: 20 µm.