Fig. 2.

In vitro properties of GE3111. The absorbance (a) and fluorescence emission spectra (b) are shown in DMSO, water, toluene, IV formulation (58.5 % distilled water, 30 % 2-hydroxypropyl-β-cyclodextrin, 10 % propylene glycol, 1 % PEG-300, and 0.5 % DMSO), and olive oil. (c) Binding of GE3111 to purified native myelin basic protein is measured by fluorescence polarization. Unbound GE3111 has an average anisotropy of 0.250. Incubation of 100 nM GE3111 with an increasing concentration of MBP resulted in an increase in anisotropy saturating at 0.321. The calculated binding affinity (K _d) is about 15 nM.